Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
Identifieur interne : 002031 ( Main/Exploration ); précédent : 002030; suivant : 002032Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
Auteurs : Long Wu [Japon] ; Akio Kumagai [Japon] ; Seung-Hwan Lee [Corée du Sud] ; Takashi Endo [Japon]Source :
- Bioresource technology [ 1873-2976 ] ; 2014.
Descripteurs français
- KwdFr :
- Biomasse (MeSH), Bois (effets des médicaments et des substances chimiques), Cellobiose (métabolisme), Cellulase (métabolisme), Diffraction des rayons X (MeSH), Glucanes (analyse), Glucose (métabolisme), Hydrolyse (effets des médicaments et des substances chimiques), Imidazoles (pharmacologie), Lignine (isolement et purification), Populus (effets des médicaments et des substances chimiques), Xylanes (analyse), Xylose (métabolisme).
- MESH :
- analyse : Glucanes, Xylanes.
- effets des médicaments et des substances chimiques : Bois, Hydrolyse, Populus.
- isolement et purification : Lignine.
- métabolisme : Cellobiose, Cellulase, Glucose, Xylose.
- pharmacologie : Imidazoles.
- Biomasse, Diffraction des rayons X.
English descriptors
- KwdEn :
- Biomass (MeSH), Cellobiose (metabolism), Cellulase (metabolism), Glucans (analysis), Glucose (metabolism), Hydrolysis (drug effects), Imidazoles (pharmacology), Lignin (isolation & purification), Populus (drug effects), Wood (drug effects), X-Ray Diffraction (MeSH), Xylans (analysis), Xylose (metabolism).
- MESH :
- chemical , analysis : Glucans, Xylans.
- chemical , isolation & purification : Lignin.
- chemical , metabolism : Cellobiose, Cellulase, Glucose, Xylose.
- drug effects : Hydrolysis, Populus, Wood.
- chemical , pharmacology : Imidazoles.
- Biomass, X-Ray Diffraction.
Abstract
This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood. Ground biomass was subjected to chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination. The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated. IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation. The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
DOI: 10.1016/j.biortech.2014.03.144
PubMed: 24755318
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.</title><author><name sortKey="Wu, Long" sort="Wu, Long" uniqKey="Wu L" first="Long" last="Wu">Long Wu</name><affiliation wicri:level="1"><nlm:affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046</wicri:regionArea><wicri:noRegion>Hiroshima 739-0046</wicri:noRegion></affiliation></author><author><name sortKey="Kumagai, Akio" sort="Kumagai, Akio" uniqKey="Kumagai A" first="Akio" last="Kumagai">Akio Kumagai</name><affiliation wicri:level="1"><nlm:affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046</wicri:regionArea><wicri:noRegion>Hiroshima 739-0046</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Seung Hwan" sort="Lee, Seung Hwan" uniqKey="Lee S" first="Seung-Hwan" last="Lee">Seung-Hwan Lee</name><affiliation wicri:level="1"><nlm:affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan; Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, Chunchen 200-701, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan; Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, Chunchen 200-701</wicri:regionArea><wicri:noRegion>Chunchen 200-701</wicri:noRegion></affiliation></author><author><name sortKey="Endo, Takashi" sort="Endo, Takashi" uniqKey="Endo T" first="Takashi" last="Endo">Takashi Endo</name><affiliation wicri:level="1"><nlm:affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan. Electronic address: t-endo@aist.go.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046</wicri:regionArea><wicri:noRegion>Hiroshima 739-0046</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24755318</idno><idno type="pmid">24755318</idno><idno type="doi">10.1016/j.biortech.2014.03.144</idno><idno type="wicri:Area/Main/Corpus">002215</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002215</idno><idno type="wicri:Area/Main/Curation">002215</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002215</idno><idno type="wicri:Area/Main/Exploration">002215</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.</title><author><name sortKey="Wu, Long" sort="Wu, Long" uniqKey="Wu L" first="Long" last="Wu">Long Wu</name><affiliation wicri:level="1"><nlm:affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046</wicri:regionArea><wicri:noRegion>Hiroshima 739-0046</wicri:noRegion></affiliation></author><author><name sortKey="Kumagai, Akio" sort="Kumagai, Akio" uniqKey="Kumagai A" first="Akio" last="Kumagai">Akio Kumagai</name><affiliation wicri:level="1"><nlm:affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046</wicri:regionArea><wicri:noRegion>Hiroshima 739-0046</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Seung Hwan" sort="Lee, Seung Hwan" uniqKey="Lee S" first="Seung-Hwan" last="Lee">Seung-Hwan Lee</name><affiliation wicri:level="1"><nlm:affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan; Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, Chunchen 200-701, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan; Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, Chunchen 200-701</wicri:regionArea><wicri:noRegion>Chunchen 200-701</wicri:noRegion></affiliation></author><author><name sortKey="Endo, Takashi" sort="Endo, Takashi" uniqKey="Endo T" first="Takashi" last="Endo">Takashi Endo</name><affiliation wicri:level="1"><nlm:affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan. Electronic address: t-endo@aist.go.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046</wicri:regionArea><wicri:noRegion>Hiroshima 739-0046</wicri:noRegion></affiliation></author></analytic><series><title level="j">Bioresource technology</title><idno type="eISSN">1873-2976</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomass (MeSH)</term><term>Cellobiose (metabolism)</term><term>Cellulase (metabolism)</term><term>Glucans (analysis)</term><term>Glucose (metabolism)</term><term>Hydrolysis (drug effects)</term><term>Imidazoles (pharmacology)</term><term>Lignin (isolation & purification)</term><term>Populus (drug effects)</term><term>Wood (drug effects)</term><term>X-Ray Diffraction (MeSH)</term><term>Xylans (analysis)</term><term>Xylose (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biomasse (MeSH)</term><term>Bois (effets des médicaments et des substances chimiques)</term><term>Cellobiose (métabolisme)</term><term>Cellulase (métabolisme)</term><term>Diffraction des rayons X (MeSH)</term><term>Glucanes (analyse)</term><term>Glucose (métabolisme)</term><term>Hydrolyse (effets des médicaments et des substances chimiques)</term><term>Imidazoles (pharmacologie)</term><term>Lignine (isolement et purification)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Xylanes (analyse)</term><term>Xylose (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Glucans</term><term>Xylans</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellobiose</term><term>Cellulase</term><term>Glucose</term><term>Xylose</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glucanes</term><term>Xylanes</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Hydrolysis</term><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Bois</term><term>Hydrolyse</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellobiose</term><term>Cellulase</term><term>Glucose</term><term>Xylose</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Imidazoles</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Imidazoles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>X-Ray Diffraction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Diffraction des rayons X</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood. Ground biomass was subjected to chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination. The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated. IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation. The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24755318</PMID><DateCompleted><Year>2014</Year><Month>12</Month><Day>28</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>162</Volume><PubDate><Year>2014</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.</ArticleTitle><Pagination><MedlinePgn>207-12</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2014.03.144</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0960-8524(14)00449-0</ELocationID><Abstract><AbstractText>This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood. Ground biomass was subjected to chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination. The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated. IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation. The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Long</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumagai</LastName><ForeName>Akio</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Seung-Hwan</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan; Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, Chunchen 200-701, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Endo</LastName><ForeName>Takashi</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan. Electronic address: t-endo@aist.go.jp.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>04</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour Technol</MedlineTA><NlmUniqueID>9889523</NlmUniqueID><ISSNLinking>0960-8524</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005936">Glucans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007093">Imidazoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014990">Xylans</NameOfSubstance></Chemical><Chemical><RegistryNumber>16462-44-5</RegistryNumber><NameOfSubstance UI="D002475">Cellobiose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>A1TA934AKO</RegistryNumber><NameOfSubstance UI="D014994">Xylose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical><Chemical><RegistryNumber>TVE1D62MAK</RegistryNumber><NameOfSubstance UI="C518739">1-ethyl-3-methylimidazolium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002475" MajorTopicYN="N">Cellobiose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005936" MajorTopicYN="N">Glucans</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007093" MajorTopicYN="N">Imidazoles</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014961" MajorTopicYN="N">X-Ray Diffraction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014990" MajorTopicYN="N">Xylans</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014994" MajorTopicYN="N">Xylose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Accessibility</Keyword><Keyword MajorTopicYN="N">Bioenergy</Keyword><Keyword MajorTopicYN="N">Ethanol</Keyword><Keyword MajorTopicYN="N">Polymorph</Keyword><Keyword MajorTopicYN="N">Saccharification</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>02</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>03</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>4</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>4</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>12</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24755318</ArticleId><ArticleId IdType="pii">S0960-8524(14)00449-0</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2014.03.144</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Wu, Long" sort="Wu, Long" uniqKey="Wu L" first="Long" last="Wu">Long Wu</name></noRegion><name sortKey="Endo, Takashi" sort="Endo, Takashi" uniqKey="Endo T" first="Takashi" last="Endo">Takashi Endo</name><name sortKey="Kumagai, Akio" sort="Kumagai, Akio" uniqKey="Kumagai A" first="Akio" last="Kumagai">Akio Kumagai</name></country><country name="Corée du Sud"><noRegion><name sortKey="Lee, Seung Hwan" sort="Lee, Seung Hwan" uniqKey="Lee S" first="Seung-Hwan" last="Lee">Seung-Hwan Lee</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002031 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002031 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24755318
|texte= Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24755318" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |